Perturbation of wood cellulose synthesis causes pleiotropic effects in transgenic aspen.
Identifieur interne : 002D63 ( Main/Exploration ); précédent : 002D62; suivant : 002D64Perturbation of wood cellulose synthesis causes pleiotropic effects in transgenic aspen.
Auteurs : Chandrashekhar P. Joshi [États-Unis] ; Shivegowda Thammannagowda ; Takeshi Fujino ; Ji-Qing Gou ; Utku Avci ; Candace H. Haigler ; Lisa M. Mcdonnell ; Shawn D. Mansfield ; Bemnet Mengesha ; Nicholas C. Carpita ; Darby Harris ; Seth Debolt ; Gary F. PeterSource :
- Molecular plant [ 1752-9867 ] ; 2011.
Descripteurs français
- KwdFr :
- Cellulose (métabolisme), Glucosyltransferases (génétique), Glucosyltransferases (métabolisme), Lignine (métabolisme), Populus (croissance et développement), Populus (génétique), Populus (métabolisme), Végétaux génétiquement modifiés (croissance et développement), Végétaux génétiquement modifiés (génétique), Végétaux génétiquement modifiés (métabolisme).
- MESH :
- croissance et développement : Populus, Végétaux génétiquement modifiés.
- génétique : Glucosyltransferases, Populus, Végétaux génétiquement modifiés.
- métabolisme : Cellulose, Glucosyltransferases, Lignine, Populus, Végétaux génétiquement modifiés.
English descriptors
- KwdEn :
- Cellulose (metabolism), Glucosyltransferases (genetics), Glucosyltransferases (metabolism), Lignin (metabolism), Plants, Genetically Modified (genetics), Plants, Genetically Modified (growth & development), Plants, Genetically Modified (metabolism), Populus (genetics), Populus (growth & development), Populus (metabolism).
- MESH :
- chemical , genetics : Glucosyltransferases.
- chemical , metabolism : Cellulose, Glucosyltransferases, Lignin.
- genetics : Plants, Genetically Modified, Populus.
- growth & development : Plants, Genetically Modified, Populus.
- metabolism : Plants, Genetically Modified, Populus.
Abstract
Genetic manipulation of cellulose biosynthesis in trees may provide novel insights into the growth and development of trees. To explore this possibility, the overexpression of an aspen secondary wall-associated cellulose synthase (PtdCesA8) gene was attempted in transgenic aspen (Populus tremuloides L.) and unexpectedly resulted in silencing of the transgene as well as its endogenous counterparts. The main axis of the transgenic aspen plants quickly stopped growing, and weak branches adopted a weeping growth habit. Furthermore, transgenic plants initially developed smaller leaves and a less extensive root system. Secondary xylem (wood) of transgenic aspen plants contained as little as 10% cellulose normalized to dry weight compared to 41% cellulose typically found in normal aspen wood. This massive reduction in cellulose was accompanied by proportional increases in lignin (35%) and non-cellulosic polysaccharides (55%) compared to the 22% lignin and 36% non-cellulosic polysaccharides in control plants. The transgenic stems produced typical collapsed or 'irregular' xylem vessels that had altered secondary wall morphology and contained greatly reduced amounts of crystalline cellulose. These results demonstrate the fundamental role of secondary wall cellulose within the secondary xylem in maintaining the strength and structural integrity required to establish the vertical growth habit in trees.
DOI: 10.1093/mp/ssq081
PubMed: 21300756
Affiliations:
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Joshi</name><affiliation wicri:level="2"><nlm:affiliation>Biotechnology Research Center, School of Forest Resources and Environmental Sciences, Michigan Technological University, Houghton, MI 49931, USA. cpjoshi@mtu.edu</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Biotechnology Research Center, School of Forest Resources and Environmental Sciences, Michigan Technological University, Houghton, MI 49931</wicri:regionArea><placeName><region type="state">Michigan</region></placeName></affiliation></author><author><name sortKey="Thammannagowda, Shivegowda" sort="Thammannagowda, Shivegowda" uniqKey="Thammannagowda S" first="Shivegowda" last="Thammannagowda">Shivegowda Thammannagowda</name></author><author><name sortKey="Fujino, Takeshi" sort="Fujino, Takeshi" uniqKey="Fujino T" first="Takeshi" last="Fujino">Takeshi Fujino</name></author><author><name sortKey="Gou, Ji Qing" sort="Gou, Ji Qing" uniqKey="Gou J" first="Ji-Qing" last="Gou">Ji-Qing Gou</name></author><author><name sortKey="Avci, Utku" sort="Avci, Utku" uniqKey="Avci U" first="Utku" last="Avci">Utku Avci</name></author><author><name sortKey="Haigler, Candace H" sort="Haigler, Candace H" uniqKey="Haigler C" first="Candace H" last="Haigler">Candace H. Haigler</name></author><author><name sortKey="Mcdonnell, Lisa M" sort="Mcdonnell, Lisa M" uniqKey="Mcdonnell L" first="Lisa M" last="Mcdonnell">Lisa M. Mcdonnell</name></author><author><name sortKey="Mansfield, Shawn D" sort="Mansfield, Shawn D" uniqKey="Mansfield S" first="Shawn D" last="Mansfield">Shawn D. Mansfield</name></author><author><name sortKey="Mengesha, Bemnet" sort="Mengesha, Bemnet" uniqKey="Mengesha B" first="Bemnet" last="Mengesha">Bemnet Mengesha</name></author><author><name sortKey="Carpita, Nicholas C" sort="Carpita, Nicholas C" uniqKey="Carpita N" first="Nicholas C" last="Carpita">Nicholas C. 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Joshi</name><affiliation wicri:level="2"><nlm:affiliation>Biotechnology Research Center, School of Forest Resources and Environmental Sciences, Michigan Technological University, Houghton, MI 49931, USA. cpjoshi@mtu.edu</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Biotechnology Research Center, School of Forest Resources and Environmental Sciences, Michigan Technological University, Houghton, MI 49931</wicri:regionArea><placeName><region type="state">Michigan</region></placeName></affiliation></author><author><name sortKey="Thammannagowda, Shivegowda" sort="Thammannagowda, Shivegowda" uniqKey="Thammannagowda S" first="Shivegowda" last="Thammannagowda">Shivegowda Thammannagowda</name></author><author><name sortKey="Fujino, Takeshi" sort="Fujino, Takeshi" uniqKey="Fujino T" first="Takeshi" last="Fujino">Takeshi Fujino</name></author><author><name sortKey="Gou, Ji Qing" sort="Gou, Ji Qing" uniqKey="Gou J" first="Ji-Qing" last="Gou">Ji-Qing Gou</name></author><author><name sortKey="Avci, Utku" sort="Avci, Utku" uniqKey="Avci U" first="Utku" last="Avci">Utku Avci</name></author><author><name sortKey="Haigler, Candace H" sort="Haigler, Candace H" uniqKey="Haigler C" first="Candace H" last="Haigler">Candace H. Haigler</name></author><author><name sortKey="Mcdonnell, Lisa M" sort="Mcdonnell, Lisa M" uniqKey="Mcdonnell L" first="Lisa M" last="Mcdonnell">Lisa M. Mcdonnell</name></author><author><name sortKey="Mansfield, Shawn D" sort="Mansfield, Shawn D" uniqKey="Mansfield S" first="Shawn D" last="Mansfield">Shawn D. Mansfield</name></author><author><name sortKey="Mengesha, Bemnet" sort="Mengesha, Bemnet" uniqKey="Mengesha B" first="Bemnet" last="Mengesha">Bemnet Mengesha</name></author><author><name sortKey="Carpita, Nicholas C" sort="Carpita, Nicholas C" uniqKey="Carpita N" first="Nicholas C" last="Carpita">Nicholas C. Carpita</name></author><author><name sortKey="Harris, Darby" sort="Harris, Darby" uniqKey="Harris D" first="Darby" last="Harris">Darby Harris</name></author><author><name sortKey="Debolt, Seth" sort="Debolt, Seth" uniqKey="Debolt S" first="Seth" last="Debolt">Seth Debolt</name></author><author><name sortKey="Peter, Gary F" sort="Peter, Gary F" uniqKey="Peter G" first="Gary F" last="Peter">Gary F. Peter</name></author></analytic><series><title level="j">Molecular plant</title><idno type="eISSN">1752-9867</idno><imprint><date when="2011" type="published">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cellulose (metabolism)</term><term>Glucosyltransferases (genetics)</term><term>Glucosyltransferases (metabolism)</term><term>Lignin (metabolism)</term><term>Plants, Genetically Modified (genetics)</term><term>Plants, Genetically Modified (growth & development)</term><term>Plants, Genetically Modified (metabolism)</term><term>Populus (genetics)</term><term>Populus (growth & development)</term><term>Populus (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Cellulose (métabolisme)</term><term>Glucosyltransferases (génétique)</term><term>Glucosyltransferases (métabolisme)</term><term>Lignine (métabolisme)</term><term>Populus (croissance et développement)</term><term>Populus (génétique)</term><term>Populus (métabolisme)</term><term>Végétaux génétiquement modifiés (croissance et développement)</term><term>Végétaux génétiquement modifiés (génétique)</term><term>Végétaux génétiquement modifiés (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Glucosyltransferases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Cellulose</term><term>Glucosyltransferases</term><term>Lignin</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Populus</term><term>Végétaux génétiquement modifiés</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Plants, Genetically Modified</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Plants, Genetically Modified</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Glucosyltransferases</term><term>Populus</term><term>Végétaux génétiquement modifiés</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Plants, Genetically Modified</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Cellulose</term><term>Glucosyltransferases</term><term>Lignine</term><term>Populus</term><term>Végétaux génétiquement modifiés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Genetic manipulation of cellulose biosynthesis in trees may provide novel insights into the growth and development of trees. To explore this possibility, the overexpression of an aspen secondary wall-associated cellulose synthase (PtdCesA8) gene was attempted in transgenic aspen (Populus tremuloides L.) and unexpectedly resulted in silencing of the transgene as well as its endogenous counterparts. The main axis of the transgenic aspen plants quickly stopped growing, and weak branches adopted a weeping growth habit. Furthermore, transgenic plants initially developed smaller leaves and a less extensive root system. Secondary xylem (wood) of transgenic aspen plants contained as little as 10% cellulose normalized to dry weight compared to 41% cellulose typically found in normal aspen wood. This massive reduction in cellulose was accompanied by proportional increases in lignin (35%) and non-cellulosic polysaccharides (55%) compared to the 22% lignin and 36% non-cellulosic polysaccharides in control plants. The transgenic stems produced typical collapsed or 'irregular' xylem vessels that had altered secondary wall morphology and contained greatly reduced amounts of crystalline cellulose. These results demonstrate the fundamental role of secondary wall cellulose within the secondary xylem in maintaining the strength and structural integrity required to establish the vertical growth habit in trees.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">21300756</PMID><DateCompleted><Year>2011</Year><Month>07</Month><Day>05</Day></DateCompleted><DateRevised><Year>2011</Year><Month>03</Month><Day>25</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1752-9867</ISSN><JournalIssue CitedMedium="Internet"><Volume>4</Volume><Issue>2</Issue><PubDate><Year>2011</Year><Month>Mar</Month></PubDate></JournalIssue><Title>Molecular plant</Title><ISOAbbreviation>Mol Plant</ISOAbbreviation></Journal><ArticleTitle>Perturbation of wood cellulose synthesis causes pleiotropic effects in transgenic aspen.</ArticleTitle><Pagination><MedlinePgn>331-45</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1093/mp/ssq081</ELocationID><Abstract><AbstractText>Genetic manipulation of cellulose biosynthesis in trees may provide novel insights into the growth and development of trees. To explore this possibility, the overexpression of an aspen secondary wall-associated cellulose synthase (PtdCesA8) gene was attempted in transgenic aspen (Populus tremuloides L.) and unexpectedly resulted in silencing of the transgene as well as its endogenous counterparts. The main axis of the transgenic aspen plants quickly stopped growing, and weak branches adopted a weeping growth habit. Furthermore, transgenic plants initially developed smaller leaves and a less extensive root system. Secondary xylem (wood) of transgenic aspen plants contained as little as 10% cellulose normalized to dry weight compared to 41% cellulose typically found in normal aspen wood. This massive reduction in cellulose was accompanied by proportional increases in lignin (35%) and non-cellulosic polysaccharides (55%) compared to the 22% lignin and 36% non-cellulosic polysaccharides in control plants. The transgenic stems produced typical collapsed or 'irregular' xylem vessels that had altered secondary wall morphology and contained greatly reduced amounts of crystalline cellulose. These results demonstrate the fundamental role of secondary wall cellulose within the secondary xylem in maintaining the strength and structural integrity required to establish the vertical growth habit in trees.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Joshi</LastName><ForeName>Chandrashekhar P</ForeName><Initials>CP</Initials><AffiliationInfo><Affiliation>Biotechnology Research Center, School of Forest Resources and Environmental Sciences, Michigan Technological University, Houghton, MI 49931, USA. cpjoshi@mtu.edu</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Thammannagowda</LastName><ForeName>Shivegowda</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Fujino</LastName><ForeName>Takeshi</ForeName><Initials>T</Initials></Author><Author ValidYN="Y"><LastName>Gou</LastName><ForeName>Ji-Qing</ForeName><Initials>JQ</Initials></Author><Author ValidYN="Y"><LastName>Avci</LastName><ForeName>Utku</ForeName><Initials>U</Initials></Author><Author ValidYN="Y"><LastName>Haigler</LastName><ForeName>Candace H</ForeName><Initials>CH</Initials></Author><Author ValidYN="Y"><LastName>McDonnell</LastName><ForeName>Lisa M</ForeName><Initials>LM</Initials></Author><Author ValidYN="Y"><LastName>Mansfield</LastName><ForeName>Shawn D</ForeName><Initials>SD</Initials></Author><Author ValidYN="Y"><LastName>Mengesha</LastName><ForeName>Bemnet</ForeName><Initials>B</Initials></Author><Author ValidYN="Y"><LastName>Carpita</LastName><ForeName>Nicholas C</ForeName><Initials>NC</Initials></Author><Author ValidYN="Y"><LastName>Harris</LastName><ForeName>Darby</ForeName><Initials>D</Initials></Author><Author ValidYN="Y"><LastName>Debolt</LastName><ForeName>Seth</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Peter</LastName><ForeName>Gary F</ForeName><Initials>GF</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2011</Year><Month>02</Month><Day>07</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Mol Plant</MedlineTA><NlmUniqueID>101465514</NlmUniqueID><ISSNLinking>1674-2052</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>9004-34-6</RegistryNumber><NameOfSubstance UI="D002482">Cellulose</NameOfSubstance></Chemical><Chemical><RegistryNumber>9005-53-2</RegistryNumber><NameOfSubstance UI="D008031">Lignin</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.4.1.-</RegistryNumber><NameOfSubstance UI="D005964">Glucosyltransferases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.4.1.-</RegistryNumber><NameOfSubstance UI="C478648">cellulose synthase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D002482" MajorTopicYN="N">Cellulose</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005964" MajorTopicYN="N">Glucosyltransferases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008031" MajorTopicYN="N">Lignin</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="Y">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2011</Year><Month>2</Month><Day>9</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2011</Year><Month>2</Month><Day>9</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2011</Year><Month>7</Month><Day>6</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">21300756</ArticleId><ArticleId IdType="pii">S1674-2052(14)60623-7</ArticleId><ArticleId IdType="doi">10.1093/mp/ssq081</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Michigan</li></region></list><tree><noCountry><name sortKey="Avci, Utku" sort="Avci, Utku" uniqKey="Avci U" first="Utku" last="Avci">Utku Avci</name><name sortKey="Carpita, Nicholas C" sort="Carpita, Nicholas C" uniqKey="Carpita N" first="Nicholas C" last="Carpita">Nicholas C. Carpita</name><name sortKey="Debolt, Seth" sort="Debolt, Seth" uniqKey="Debolt S" first="Seth" last="Debolt">Seth Debolt</name><name sortKey="Fujino, Takeshi" sort="Fujino, Takeshi" uniqKey="Fujino T" first="Takeshi" last="Fujino">Takeshi Fujino</name><name sortKey="Gou, Ji Qing" sort="Gou, Ji Qing" uniqKey="Gou J" first="Ji-Qing" last="Gou">Ji-Qing Gou</name><name sortKey="Haigler, Candace H" sort="Haigler, Candace H" uniqKey="Haigler C" first="Candace H" last="Haigler">Candace H. Haigler</name><name sortKey="Harris, Darby" sort="Harris, Darby" uniqKey="Harris D" first="Darby" last="Harris">Darby Harris</name><name sortKey="Mansfield, Shawn D" sort="Mansfield, Shawn D" uniqKey="Mansfield S" first="Shawn D" last="Mansfield">Shawn D. Mansfield</name><name sortKey="Mcdonnell, Lisa M" sort="Mcdonnell, Lisa M" uniqKey="Mcdonnell L" first="Lisa M" last="Mcdonnell">Lisa M. Mcdonnell</name><name sortKey="Mengesha, Bemnet" sort="Mengesha, Bemnet" uniqKey="Mengesha B" first="Bemnet" last="Mengesha">Bemnet Mengesha</name><name sortKey="Peter, Gary F" sort="Peter, Gary F" uniqKey="Peter G" first="Gary F" last="Peter">Gary F. Peter</name><name sortKey="Thammannagowda, Shivegowda" sort="Thammannagowda, Shivegowda" uniqKey="Thammannagowda S" first="Shivegowda" last="Thammannagowda">Shivegowda Thammannagowda</name></noCountry><country name="États-Unis"><region name="Michigan"><name sortKey="Joshi, Chandrashekhar P" sort="Joshi, Chandrashekhar P" uniqKey="Joshi C" first="Chandrashekhar P" last="Joshi">Chandrashekhar P. Joshi</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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